Novel synthesis of 5-((4-phenylpiperazin-1-yl) methyl)-1H-pyrrole-2-carbaldehyde metal-complexes using Mannich reaction and investigate their cytotoxicity against MCF-7 cell line

Abstract

An acidic proton next to a carbonyl functional group undergoes a multicomponent process known as the Mannich reaction, which results in aminoalkylation. It requires a suitable carbonyl molecule, such formaldehyde, and either a primary, secondary, or ammonia amine. in this study mannich reaction was used to prepare 5-((4-phenylpiperazin-1-yl) methyl)-1H-pyrrole-2-carbaldehyde (L1) through the reaction between Phenylpiperazine react with formalin and pyrolcarbaldehyde. the (L1) ligand furthermore react with, Ni and Pd to produce C2 and C3 complexes respectively. the prepared compounds were characterized by elemental analysis, ¹HNMR, ¹³CNMR spectroscopy and FTIR spectroscopy. the anti-cancer activity of these compounds were investigated invitro at different concentrations against breast cancer MCF-7 cell line, the results show that the C2 and C3 achieved 72.6 and 57.15% cytotoxicity in MCF-7 cell line.