Evaluation of the effects of ZnO NPs biosynthesis on Gene Expression for Hemolysin Hla Gene of S. aureus Bacteria Isolated from Diabetic Foot Ulcer

Abstract

The clinical isolates of Staph aureus used in the current study are from (diabetic foot patients) diagnosis results of S. aureus gram positive bacteria, beta hemolysis on blood agar, mannitol fermenter and positive catalase and coagulase. Utilizing a scanning electron microscope, fourier transforms infrared (FTIR), X-ray diffraction (XRD), and UV-visible spectrophotometer, were able to characterize the physical-chemical composition of ZnO NPs produced during the biosynthesis process (SEM). According to the XRD results, they were very small crystals with a majority spherical form, measuring 13.5 nm in size. Using the agar well diffusion method and optical densities data at 630 nm, the minimum inhibition concentration, or MIC test, of ZnO NPs at concentrations (31.25 to 500) g/ml against bacterial isolates have been established. Results of the best MIC trial with 125g/ml ZnO NPs concentration revealed a significant difference in the anti-bacterial inhibition for isolates under study at P<0.01. Using the RT-qPCR method and the 16SrRNA gene as a reference, the expression of the Hla gene was examined both before and after exposure to ZnO NPs. Cycle threshold (Ct) values for the Hla gene and gene expression of the isolates were found to differ significantly at P<0.05, according to the data. According to the study's findings, biosynthesized ZnO NPs with a concentration of 125 g/ml had inhibitory effects on bacterial isolates and had the potential to downregulate gene expression.