Bean Seed Cover Agar, New Culture Medium For Cultivation and Identification of Cryptococcus Neoformans

Abstract

Melanin production by Cryptococcus neoformans is widely used for isolation and identification of C. neoformans. In this study, we used the new medium prepared from fava bean seed cover extract. The isolates of C.neoformans and Candida albicans (as negative control ) were cultured on fava bean seed cover agar, Also Sunflower Seeds agar (Helianthus Annuus) inoculated with C. noformans as positive control. results showed that at 48 hours the isolates of C. Neoformans was pigmented on fava bean agar and produce dark brown colonies, while C. Albicans grow, but don’t produce pigment. The analysis of seed cover by high performance liquid chromatography (HPLC) showed that the fava bean seed cover content: Myrecelin, Ferrulic acid, Caffeic acid, Ascorbic acid, Apigenin and Kaempferol.The present phenolic compound confirms fava bean seed cover agar useful as a media for the rapid identification of C. neoformans.