Effect of Level of Tumor Necrosis Factor-Alpha (TNF-Α) And Follicle Stimulating Hormone (FSH) Impacts on The Size of The Ovarian Follicles of Local Cows

Abstract

This study was conducted in the laboratories of the Department of Animal Production Techniques at the Technical College in Mussaib (TCM) from September 2021 to January 2022 to study the relationship between Tumor Necrosis Factor-alpha (TNF-α) and Follicle Stimulating Hormone (FSH) with ovarian follicle size of local cows. 150 reproductive systems were collected from non-pregnant local cows, which were slaughtered in the abattoirs of Babylon Province and were transported to the laboratory within two hours. Ovaries were removed (300 ovaries) and their dimensions were measured. The follicles were measured and classified into three categories. Follicular fluid was drawn from the visible follicles (2-8) on the surface of each ovary and placed in an Eppendorf tube to measure the concentration level Tumor Necrosis Factor-alpha (TNF-α) and Follicle Stimulating Hormone (FSH) in the follicular fluid. The results showed a significant (P<0.01) increase in the average level of Tumor Necrosis Factor-alpha (TNF-α) in the follicular fluid with an increase in follicular volume and a decrease in large follicles. Also, there was a significant impact (P<0.01) in the average level of Follicle Stimulating Hormone (FSH) in the follicular fluid for the groups of medium and small size of the follicles. It can be concluded that the levels of Tumor Necrosis Factor-alpha (TNF-α) and Follicle Stimulating Hormone (FSH) are impacted by the size of the ovarian follicle.