Sequencing Report of Cox1 Gene-Based Amplicons in Ten Isolates of Silver Carp

Abstract

One specific PCR fragment partially covering the coding regions of the cytochrome c oxidase subunit I encoding gene was selected in this study. The amplified fragments were directly exposed to Sanger sequencing experiments to assess the pattern of genetic polymorphism in the collected fish samples. Then, a specific comprehensive tree was generated to assess the accurate identification of the observed variants and their phylogenetic distribution Sequencing reactions showed the accurate identity of the investigated samples due to the presence of one species, as it was confirmed that S11-S20 belonged to silver carp (Hypophthalmichthys molitrix). Further details were also observed from the investigated sequencing reactions for the investigated S11-S20 samples. No nucleic acid variations were identified in the silver carp isolates. The generated phylogenetic tree was constructed to incorporate five phylogenetic clades with distinct phylogenetic distances. It was inferred from the tree that the silver carp group was suited in the vicinity to the clade of Prussian carp. This positioning referred to the fact that both species shared the highest homology than the other incorporated outgroup species.