Preparation Of Some Hydroxamic Acid Derivatives and Study of their Biological Activity as Anti-Cancer and Anti-Bacterial Agents

Abstract

In this research, the following hydroxamic acid compounds, N1, N2 -dihydroxyphthalamide and N1, N3 -dihydroxymalonamide, were prepared. And that is through the direct interaction between the primary esters of these compounds with hydroxylamine hydrochloride and using sodium hydroxide as a base medium PH (12-13) and the formation of hydroxamic acid was detected using the ferric ion as a primary reagent, and we infer that through the appearance of a pinkish-red color. The compounds after reacting with acetyl chloride to the following derivatives N 1, N2 -Di Acetyl phtalamide, N 1, N2 -Di Acetyl malonamide, these compounds were purified using the recrystallization process, and the formation of these compounds was also detected using the ferric ion, as there was no change in the color of the solution, as well as the melting points were set and these were diagnosed The compounds prepared using the spectrum of FT.IR)) (H.NMR), and (C13 NMR). The biological activity of these prepared compounds N1, N2 -Di Acetyl phtalamide, N1, N2 -Di Acetyl malonamide was studied by using tissue culture of cell cultures. breast cancer type (MCF-7) By promoting this type of cancer cells in a culture medium (ROMI-1640) and using the compound (MTT) method, these compounds gave high efficacy against cancer cells at a concentration of less than (50 micromoles) and a value of TC 50 = 26.85 mg/ml for compound N 1 , N2 -Di Acetyl phtalamide with a value of TC 50 = 22.52 mg/ml for the compound, N 1, N2 -Di Acetyl malonamide. The results showed that derivatives of hydroxamic acids are powerful against cancer cells, especially breast cancer type (MCF-7), and the effect of these compounds has also been studied. prepared on two types of positive and negative bacteria (E scherichiacoli Staphylococcus), where this study showed that the prepared compounds had a high inhibition of bacteria at a concentration (500 mg/ml).