Analysis of Urine Type IV Collagen in Diabetic Nephropathy Patients

Abstract

Type 2 diabetes mellitus is a disease characterized by hyperglycemia (increased blood sugar levels). Hyperglycemia from diabetes mellitus affects many organs of the body, causing complications, one of which is diabetic nephropathy.Type IV collagen is a major component of the glomerular basement membrane (GBM) and is mesangial, the level of type IV collagen in a urine sample reflects the rate of matrix turn over in the injured kidney.Urinary Type Iv collagen recently emerged as a marker for the early detection of diabetic nephropathy. This research aimed to measure and to compare urine type IV collagen levels in patients beetwen non diabetic nephropathy and diabetic nephropathy. The research method used was the observational analytic with cross sectional design. The data in this research used 50 samples, of which 25 samples were from diabetic nephropathy and 25 samples were from non diabetic nephropathy. Type IV collagen levels were examined using the ELISA method. The results showed that the results of the statistical analysis of urine type IV collagen comparison test in nephropathy and  non nephropathy is 26.28 ng/mL and 12.50 ng/mL. Correlation test resuts of urinary tipe IV collagen levels ACR urine is 0.536 (p≤0.05). The conclusion is that there are differences in urinary type IV Collagen in nephropathy and non nephropathy patients, next there is a correlation between urinary ACR and urinary type IV collagen in diabetic nephropath.