Genotype Distribution of High Risk Human Papillomavirus in Patients with Breast Cancer

Abstract

Breast cancer is the most common cancer diagnosed in women, accounting for more than 1 in 10 new cancer each year. It is the second most common cause of death from cancer among women in the world. Viral infections have been accounted for approximately 18–20% of breast cancer. This makes the human papilloma virus (HPV) a strong candidate for initiation and development of breast cancer. High-risk HPV E6 and E7 early oncoproteins were shown to be involved in cell-cycle progression, cellular transformation, and cancer development. In this study, 62 Formalin fixed paraffin-embedded tissue (FFPE) and 40 fresh biopsies were collected from women at different stages of breast cancer. The prevalence of HPV specific DNA in women with breast cancer was detected in 16% of FFPE samples and in 20% of fresh biopsies using nested PCR. When molecular genotyping was carried out using a multiplex PCR, it appeared that HPV-16 was the predominant genotype in 94% of positive cases of breast cancerin both sample types(FFPE and fresh tissues).