The study of relationship of Advanced Oxidation protein products and some hormones with uterine disturbances

Abstract

The study was conducted on 100 of Dairy cows in Tag-Alnahrain station sited in Al- Qadysia province post parturition (after 40-60) day after calving. The aim of study was to test the relationship between oxidative stress particularly protein oxidation and uterine health during the puerperium. The cows classified into four groups according to intense of clinical signs of merits. and neutrophil percentage in uterus and vaginal mucus discharge, in this study the cut off value of PMN %.was set at 8% to distinguish between degrees of inflammation.the group (H) was (54). healthy cows,(SCE) was 22 cows infected with subclinical endometritis, (EM1) was (11) cows infected with clinical endometritis Grad 1 and (EM2) group was (13) cows infected with clinical endometriosis Grad 2 the Advanced oxidation protein product (AOPP) was estimated in plasma and vaginal mucus discharges by using commercial laboratory method to detect the dityrosin formation which is the major content of AOPP. AOPP the good marker of protein oxidation were visualize by western blot (BCA Protein Assay Kit; Pierce Biotechnology, Rockford, LL, USA) analysis ( Witko- sarsat etal, 1996). the study demonstrate that there was increasing in plasma AOPP in EM2 group in significant value of p≤0.01, Also the study show significantly increase in the concentration of AOPP in vaginal mucus discharges in group of animal infected with (SCE) the study clarified that there was increasing in neutrophil pointage over than 8% in vaginal mucus discharge after collecting mucus by metrecheek in(EM1) group in significant value of p≤0.0l while it was high significantly(p≤0.01) in uterine mucus collected by cytobrush. The study proved positive correlation between AOPP concentration and progesterone while its correlate negatively with estrogen both effect impaired fertility by increasing the interval period and repeat breeder leading to increase the number of serves to and also the open day of dairy cattle infected by SCE in significant value of p≤0.01 the study clarified that there was obvious effect for merits on decreasing milk production period and the mean of milk product ∕ day and total of milk product in lactation period this decline was higher significantly p<0.01 in dairy cows with high infected with merits. the study proved that oxidative stress and protein oxidation antigenic effect of AOPP in uterine was a reason and biomarker impaired fertility in dairy cow infected with merits.