Synthesis of Silver Nanoparticle and Study the Effect on Adenosine Amino Hydrolase (ADA) Enzyme Activity in Blood Serum

Abstract

Chemical reduction method was used successfully to prepare cubic Ag nanoparticles using trisodium citrate as a reduction agent, all of the materials' ingredients were produced in double distilled water. A UV–vis spectrophotometer, X-ray diffraction (XRD) and Transmission electron microscopy (TEM) were used to analyses the silver NPs. The average size of Ag nanoparticles generated was 8 nm to 42 nm, according to TEM studies. The effect of Ag NPs on the activity of Adenosine amino hydrolase (ADA) in the serum of atherosclerosis patients was studied. Patients with atherosclerotic disease with and without Ag nanoparticles exhibited significantly higher serum levels of ADA activity (P < 0.01) as comparative to the healthy group. Individuals with atherosclerotic disease who obtained Ag nanoparticles had significantly reduced blood levels of ADA activity than those who did not obtained Ag nanoparticles.