Immunity of Outer Membrane of Salmonella Typhi in Rabbits

Abstract

Objectives: The release of various types of membrane vesicles is widespread, from prokaryotes to more complex eukaryotic cells. It has been known for decades that Gram‐negative bacteria shed outer membrane vesicles (OMVs), but they are also released by other groups such as Gram‐positive bacteria. There is increasing interest in vesicles and recruitment of the immune system for medical applications which could be linked to the finding that 60% of the available pharmaceutical drugs exert their effect through interaction with membrane proteins A few years after their discovery, research started to explore OMVs as vaccine product, in particular for the application against many diseases. They present a range of surface antigens in a native conformation and have natural properties like immunogenicity, self‐adjuvation and uptake by immune cells which make them attractive for application as vaccines against pathogenic bacteria. The aim of this research as determination effect of o outer membrane protein of salmonella on some immunological parameters on rabbits’ animals. Methodology: in this research This study prepare Outer membrane protein (OMPs) and Molecular Weight of Outer membrane protein (OMPs) was determinated by SDS- page, The animals was injected with 0.1ml outer membrane of intradermal of skin, As for the control animals, they were injected with normal saline and the same amount, and recording the observed skin change after (4,24,48,72) hr. in comparison with control animals. Use this test to find out the effect of outer membrane on stimulating the cellular response of rabbits, This study also determinated Interleukin (1β, 2 and17) concentration Results: the results of present study exhibited The SDS-PAGE OMP analysis of Salmonella typhi revealed the molecular weight (MW) of the gels was estimated at 44KD by comparison with standard MW markers, the present study also investigated how outer membranes influenced on the skin sensitivity and cell immunity of the rabbit. The results of the production of cytokines were estimated using the equation from the standard curve carried out with the same test. Statistic analysis on IL-2 showed a mean value (38.548±4.771,49.781±5.264 and 72.410±4.221) of the control group when compared to the immunized group with OMPs antigen and mean value (23.434±2.501) respectively. Conclusion: the outer membranes of Salmonella typhi influenced on the cell immunity of the rabbit