The effect of Pimpinella anisum extracts on the activity of a protease enzyme isolated from clinical samples of bacteria

Abstract

Background This study included the use of medicinal herbs in the treatment of many infections, especially urinary tract infections, due to the presence of great resistance to antibiotics. Anise plant extract was used in this study to inhibit the virulence factors of some types of bacteria isolated from pathological cases. Methodology The Ramadi Teaching Hospital collected 30 scientific specimens from a variety of sources, including burns, wounds, and UTI infections. These samples were collected using sterile cotton swabs. A sterile container was used to collect samples from patients with urinary tract infections, protease activity Casein substrate solution and bacterial growth supernatants were mixed together and incubated at 37°C for 20 minutes to measure the activity of the enzyme protease. A 3 ml injection of 5 percent Trichloroacetic acid (TCA) stopped the reaction, and the sample was centrifuged at 2500 rpm for 20 minutes. The experiment was set up using the same protocols except for the addition of TCA reagent before the supernatants, and the absorbance at 275 nm was then measured. Result the effect of anise extract on inhibiting the activity of some types of gram-positive bacteria such as staphylococcus and gram-negative bacteria such as Pseudomonas aeruginosa. In the activity of the protease enzyme produced by staphylococcus bacteria 16%, while the same concentration of the same extract gave the highest inhibition in the activity of the enzyme in Pseudomonas aeruginosa, reaching 25%, the analysis of variance for the effect of anise plant extract on gram-positive and gram-negative bacteria. There were significant differences regarding the effect of this extract on gram-negative and gram-positive bacteria. There were significant differences for the concentrations used for this extract in inhibiting the activity of this enzyme