Determination of salivary IgA (sIgA) levels in saliva of patient with dental caries

Abstract

Using the ELISA test, the content of saliva IgA (sIgA) in the saliva of the patient with dental caries was determined, as 85 samples were collected for the current study from patients aged (20-40) years out of (60) clinical samples of saliva. 25 saliva samples were collected from healthy individuals as a control group and the results were 60 (70.59)% patients with severe dental caries and 25 (29.41)% of healthy subjects. Their ages ranged from (20-50) years with a mean age of (33.75 ± 7.501) for dental caries patients while the mean age of health was (28.16 ± 8.05).  found significant differences (P ≤ 0.05) in patients with dental caries between female 42 (70)% while 18 (30)% in males. The Immunological study to determination  (sIgA) concentration  in saliva of dental caries  patients By using enzyme-linked immunosorbent assay (ELISA) technique, The result shows significant decrease the concentration  in Three markers  in dental caries cases than control ones, P≤0.001. It indicates that biomarkers sIgA, have correlation with age groups of dental caries patients with a reverse relationship between level of  sIgA in saliva with progress of age group .