Molecular Detection of sprE, fsrA and cpd Genes among E. faecalis Isolates

Abstract

Patients who were hospitalized and examined at Baghdad's Al-Karama Hospital and Medical City Hospital over the course of a three-month period (May to July 2022) supplied urine and vaginal specimens to the study's 200 participants as part of the research. At a temperature of 37 degrees Celsius, the samples were grown in a variety of media for 18–24 hours. After that, they were incubated at a temperature of 37 degrees Celsius for 18 to 24 hours on a variety of selective medium (Chromo Agar Medium). When we originally set out to determine who E. faecalis, researchers used colony morphology, microscopic investigations, and biochemical tests as their primary methods of investigation. However, only 44 (22%) of the isolates were related with E. coli, despite the fact that all 200 clinical samples cultured positive. faecalis. All previously identified isolates' DNA was taken and used in conventional PCR to amplify the ddl gene, the results of gel electrophoresis, which demonstrated that, each of the 44 isolates (100%) had, in fact, generated the same 941bp DNA fragment when run alongside a ladder. All E. faecalis isolates were subjected to cpd gene molecular investigations utilizing targeted PCR markers. 36(81.8%) of the 44 E. faecalis isolates tested positive for the presence of a cpd gene with a long length (782bp). For gene identification of sprE, a specific PCR primer was utilized. Only 12 (27.2%) of the 44 E. faecalis isolates tested positive for the sprE gene, with a long length of 591 base pairs (bp). Among 44 E. faecalis isolates tested for the presence of the fsrA gene, only 10 (22.7%) were found to be positive, when the (474 bp) band was compared to the allelic ladder, positive findings were obtained for the fsrA virulence gene.