Molecular Detection of Some Genes Responsible for Biofilm Formation in Acinetobacter Baumannii Isolated from Different Sites of Infection

Abstract

baumannii is a G -ve bacteria, non-lactose fermenter, its increasingly recognized as a major pathogen causing infections. Methods: Fifty- six of A.baumannii was isolated from 100 samples of different sources (sputum, burns, bed sore , blood, wound and urine) in both sexes. These isolates were diagnosed by VITEK -2 system. Microtiter plate method used to detect biofilm formation. DNA extraction of 56 A. baumannii isolates, then molecular detection of some biofilm genes ( bfmR and bfmS) were done by polymerase chain reaction. Results: The highest percentage of A. baumannii isolation (42%) was from sputum. These Isolates were divided into three groups according on biofilm production: strong, moderate, weak biofilm formation .Detection of biofilm formation genes revealed 38 (67.86%) positive bfmR gene versus 18 (32.14%) negative result. As well as 49(87.50%) Those isolates had the gene. bfms versus 7 (12.50 %) not carried the same gene.