Effect of Eruca Sativa extract, some heavy metals (Pb, Ni, Cr, and Al), on some soil bacterial isolates

Abstract

This study was performed in laboratories of Al-Mustansiriya University / College of Science - Department of Life Sciences during the period from 2021 / 3 / 3 till 2022 /3 / 28 to study the effect of some heavy metals on some microscopic soil organisms. The study included isolation and identification of bacteria from twenty-four samples of elements contaminated soil, the bacterial identification contained macroscopic and microscopic examinations, as well as biochemical tests, the identification then confirmed by VITEK2 system. The ability of biofilm formation of these identified bacteria was performed by using Congo red agar (CRA). The antimicrobial effects of four selected heavy metals (Al, Cr, Ni, and Pb) on the isolated bacterial isolates. Results of bacterial isolation showed that thirteen bacterial isolates were identified (three isolates of Pseudomonas aeruginosa, two isolates of Sphingomonas paucimobilis, and Stenotrophomonas maltophilia, and one isolates of each Staphylococcus auras, Staphylococcus lents, Serratia ficaria, Dermacoccus nishinomiyaensis, Kocuria rhizophila, and Kytococcus sendertarius), with isolation percentage equal to (54.11%). Results of three concentrations of plant extract (300, 150, 75 mg/ml) showed there wasn’t any antimicrobial effects of that extract against any of the isolated bacteria, with no inhibition zone of all the prepared concentrations.