Efficacy of Silver Nanoparticles Synthesized from Pineapple Fruit Extracts Against Enterococcus Faecalis and Streptococcus Sanguis (An in Vitro Study)

Abstract

Aims of the study: the study aimed to estimate the antibacterial efficacy of biosynthesizing Ag nanoparticles (Ag Nps) from pineapple extracts against some oral bacteria. Material and methods: The aqueous and ethanolic extracts were prepared from pomace and core tissues of pineapple fruit separately and the Ag Nps were synthesized by the addition of 5 mL of 1mMolar of an aqueous solution of silver nitrate waiting for color change from transparent to black, the UV Spectrophotometer, EDX, SEM, and FTIR were used to characterize Ag Nps, turbidity method was carried out for the antibacterial activity and the absorbance of 1/1,1/2 and 1/4 dilutions of the four extracts against oral bacteria [Enterococcus faecalis and Streptococcus sanguis] was measured at 530 nm by vis-spectrophotometer. Results: Ag Nps were synthesized and characterized, and all the prepared extracts and the nanoparticles had an antibacterial effect against Enterococcus faecalis and Streptococcus sanguis, the best antibacterial effects of Ag Nps biosynthesized from aqueous extract for both pineapple tissue pomace and core extract and ethanolic pomace extract (0.00) followed by 0.114 ethanolic core extract against Streptococcus sanguis, .022 ethanolic pomace extract against Streptococcus sanguis and 0.04 for ethanolic core extract against Enterococcus faecalis.Conclusion: The results showed the sensitivity of Enterococcus faecalis and Streptococcus sanguis to biologically synthesized nanoparticles made from the aqueous and ethanolic extracts, respectively, as well as the sensitivity of both types of bacteria to the aqueous extract of pineapple more than the ethanolic extract.