Antibacterial Activity of Magnesium Oxide Nanoparticles Against XDR Pseudomonas Aeruginosa Isolated from Burn Infection.

Abstract

This study was intended to study the biological activity of magnesium oxide against XDR P. aeruginosa isolated from burns infection. Seventy-Five samples and inquiries were carried out between August 2022 and December 2022. Burn infection samples were obtained from two separate hospitals in the province of Najaf (AL Hakeem hospital and Central Laboratory in najaf). Isolates were identified based on the morphological and microscopic examination, and VITEC. The results showed bacilli were the most common P. aeruginosa (52%) followed by Klebsiella pneumoniae that the most common Gram-positive was S. aureus (20%), while Gram-negative (12%), Escherichia coli (8%) , Burkholderia cepacia (4%), Acinetobacter baumannii (2.67%) and other in (1.33%). Molecular study used ERIC-PCR for the detection of phylogenetic diversity of P. aeruginosa isolated from burned samples, In general, 20 isolates characterized using the ERIC molecular techniques had number of bands with some degree of polymorphism using conventional PCR. one efficient isolate (S 10) was selected from screening different strains (36) of probiotics based on antibacterial activity. Isolate S10 was identified as Lactobacillus acidophilus depending on morphology, microscopic examination, and VITEK. The study showed the inhibitory ability of L. acidophilus all teste isolate showed high anti-biofilm activity against P.aeruginosa studied bacteria.