Impact of Bacterial Infection and Cytokines Imbalance on Pathophysiology Burns Infections

Abstract

Burns create traumatic injuries that are unpredictable and deadly which are linked to high morbidity and mortality among affected patients. So, this study aimed to measure dynamic shifts in the levels of IL-23 and IL-17 and association to a bacterial infection in the pathophysiology of burn infection. A case-control study for 60 patients suffering from burn during March-July 2021 entered the specialized burn center in middle Euphrates hospital in the Iraqi province of Al Najaf. Swabs from central burn cultured on Chromogenic medium and cetramid for 24h at 37 ℃. Two ml blood sample were taken from all patients and from 30 apparently healthy subjects as control group to evaluate IL-17 and IL-23 levels. Our results found that 28 (70%) of patients with injury > 50%, 20 isolates related to P. aerogenosa, 12 isolates for S. aureus as most common bacterial agents. Immunological study explains that IL-17 and IL-23 concentration in burn P. aeroginosae infected patients were (129.22±32.47, 114.09±11.56 pg/ml, respectively), in burn sterile patients (98.13±13.71, 192.64±28.70 pg/ml, respectively) in comparison to healthy group (18.32±4.18, 16.50±2.34 pg/ml) with significantly differences at p≤ 0.0. In conclusion: burn infection with antibiotic-resistant bacteria and persistent elevation a pro-inflammatory cytokines lead to the development of pathophysiological burn and suggest that using anti-IL-23 or IL-17 may help improve burn treatment, prevent complications, and reduce the length of hospital stay.