Molecular detection of MRSA isolated from female Patients in Al-Diwaniya city

Abstract

Staphylococcus aureus may be a gram-positive infective agent that is main reason of loads of diseases like toxic shock syndrome syndrome, pneumonia, food poisoning, and coccus scaled-skin syndrome in Iraq. Staphylococcus aureus strains are showing proof against many generations of antibiotics, significantly methicillin-resistant strains (MRSA). yet, no enough previous studies were conducted to research the biological science aspects of enetrotoxins in clinical isolates of Staphylococcus aurus in patients in Iraq.the study was conducted in AD Diwaniyah teaching hospital . Patients diagnosed cancer who had cellulitis, abscesses in addition as soft tissue infections were enclosed within the study. Staphylococcus aureus were cultivated and diagnosed . Polymerase chain reaction was used to identify the antibiotic susceptibility test and enterotoxins genes. Our findings revealed that 30 S.aureus isolates were obtained from 50 specimens, including 8 (26.6%) from cellulitis, 10 (33.3%) from abscesses, and 12 (40%) from other soft tissue infections. Antibiotic resistance was confirmed in S.aureus isolates. Seb genes were found in 21 (48%) of 25 S.aureus isolates. We concluded that isolated Staphylococcus aureus from patients had the ability to produce enterotoxins and antibiotic resistance.