Identification of Enterococci Species Isolated from Clinical Specimens by Internal Transcription Spacer (ITS) and Trna Intergenic Spacer.

Abstract

With the prevalence of enterococci that are resistant to various medicines and cause a variety of clinical illnesses in Iraqi hospitals, accurate and speedy detection and characterization of enterococci types has become critical. As a result, we used the internal transcriptional spacer region (ITS) and the tRNA gene to molecularly describe Enterococcus spp. isolated from several clinical sources (urinary, vaginal, diarrhea, spinal cord fluid). For the rapid identity of Enterococcus spp. show the genetic result different bands profiles, which indicated one to four DNA fragments. Using the PCR-ribotyping approach to identity 34 Enterococcus spp isolates according to 16S-23S rRNA (ITS) and tRNA gene findings, four genotypes, and four subtypes were discovered. The DNA fragment sizes in the 16S-23S rRNA (ITS) gene ranged from 300 to 800 bp, whereas the DNA fragment sizes in the tRNA gene ranged from 290 to 700 bp. Interspecies genetic variety was discovered through the examination of DNA band patterns. As a result, the common bands in all Enterococcus spp isolates are the widths 300 and 400 bp in 16S-23S rRNA (ITS) and 290 to 700 bp in the tRNA gene. Genotype I was the most common, accounting for 82.35 percent (28 isolates) in E. faecalis, 8.82 percent (3 isolates) in E. faecium, 5.88 percent (2 isolates) in E. casseliflavus, and 2.92 percent (1 isolate) in E. gallinarum. Many Enterococcus species are cause clinically important opportunistic infections that require precise and early identification in order to get focused treatment. Currently, the PCR-ribotyping technique and method based on sequencing for the 16S–23S rRNA (ITS) gene and the tRNA gene have proven to be a quick, accurate, and reliable method for identifying Enterococci species. PCR amplified of the 16S–23S rRNA (ITS) gene and the tRNA gene revealed 28/34 (82.35%) Enterococcus faecalis isolates, with 10/10 (100%) isolates from urine, 11/11 (100%) isolates from the vagina, 10/4 (40%) isolates from diarrhea, and 3/3 (100%) isolates from the CSF. Following that are 3/34 (8.82%) Enterococcus faecium isolates, which comprise 3/10 (30%) isolates from diarrhea. Then there were 2/34 (5.88%) Enterococcus casseliflavus isolates and 1/34 (2.94%) Enterococcus gallinarum isolates from diarrhea. We conclude that employing PCR-ribotyping to amplify the 16S-23S rRNA (ITS) and tRNA genes of pathogen enterococci provide a valid method for species identification of pathogen enterococci in clinical specimens.