Detection (Icaa, Icab, Icac ,Icad ) Genes of Staphylococcus Aureus Isolated from Diabetic Wound Infections

Abstract

Staphylococcus aureus' ability to develop biofilm and adhere to surfaces helps it be more resistant to antibiotics. The goal of this study is to look at the frequency of many genes that are involved in development (icaABCD genes) using PCR, diabetic wound infections using PCR, and biofilm formation using the microtiter plate method. Out of 164 swab samples obtained from diabetic wound infections, 48 were positive for S. aureus utilizing identification morphological and biochemical tests as well as diagnosis by the VITEK2 system. The findings revealed that (48) 100% of isolates were positive for (icaA, icaB, icaD) genes and (56.25%) for the icaC gene, indicating that isolates of S. aureus had varied biofilm formation abilities, with the presence of biofilm-related genes clearly showing the roles for ica genes as biofilm producer markers.