The Effect of Interference of Acetate and Phosphorous on the Growth of Some Chemical Characteristics and the Inhibition of Types of Pathogenic Bacteria of Ocimum Basilicum Vare Viride

Abstract

The research included studying the effect of four levels of acetic acid (0, 2, 50, 75 mg. L), which has a symbol (A0 , A1, A2, A3) respectively with four levels of phosphorous (Diphosphate, K2PO4) (0, 3, 6, 8 mg. L) and symbolized by (P0, P1, P2, P3) respectively. And their interactions. In the content of flavonoids, tannins, and phenols in leaves, and measuring the inhibitory activity of basil extract in two types of pathogenic bacteria Staphylococcus aureus, positive for Gram-negative Escherich coli . The results indicated that acetate spraying had a significant superiority in the content of a flavonoids ,tannins and phenols . The spraying at A3 level gave the highest rate of (28.48 , 34.03 and 44.17) mg.gm-1 fresh weight, respectively . Also the A3 level had a significant effect to inhibit positive and negative bacteria, as it got an antagonistic effect of the extract towards bacteria, which reached (23, 24.25) mm For Staphylococcus aureus and E.col Respectively, and the effect of phosphorous spray was significantly superior to the leaves content of flavonoids, thanes and phenols, it reached the highest at the P3 level (29.44, 35.93 and 45.14) mg.gm-1 fresh weight, respectively. Phosphorous spray had a significant effect on inhibiting positive and negative bacteria, and the highest inhibition rate at P3 level was (19.75 and 21) mm for Staphylococcus aureus and E.coli, respectively. The results of the experiment showed that the interaction between the two experimental factors had a significant effect on the leaves content of male traits. The highest effect rate was at the level A and P3 and it reached (22.45, 36.9 and 45.6) mg.gm-1. The level of A3P3 was a significant superiority in inhibiting pathogenic bacteria, as it reached (24, 26) for Staphylococcus aure and E.coli, respectively.