Application of Trifluoperazine Hydrochloride as a Colorimetric Reagent for Quantitative Sulfadiazine Drug Assay and Use in Various Infection Treatment Preparations

Abstract

A spectrophotometric method using direct, rapid and sensitive spectrophotometry of sulfadiazine SAD was determined by developing a stable and water-soluble product with a maximum absorption at 501nm by oxidative coupling reaction with Trifluoperazine hydrochloride TRF reagent. With sulfadiazine SAD and using potassium periodate KIO4, which acts as an oxidizing agent in an acidic medium in the presence of hydrochloric acid HCl, depending on the concentration of sulfadiazine SAD and according to Beer's law, the concentration range was 2-24 g.ml-1, and the molar absorbance was 9.74 x103, detection limit (LOD) was 1.22 quantity limit (LOQ) was 2.54 a relative standard deviation was 0.82 to 1.19 and the recovery was 100.4, method proposed has been effectively used for the determination of sulfadiazine.