Immuno Histo Chemistry of P63 Expression in Women Patients with Cervical Carcinoma in Baghdad City

Abstract

Background and objective :- P63 is a member of the p53 gene family. It plays an essential role  in  the  normal  development  of  the  cervix in addition  p63 is considered to be an important differentiation regulation transcriptional factor, but the roles of p63 in many epithelial tumourigenesis and metastasis processes are still not clear. This study was designed to investigate the expression of p63 in squamous cell cancer tissues of uterine cervix and determined any associated between presence of  high risk of human papilloma virus and expression of p63 in cervical carcinoma among Iraqi women patients. The expression of p63 was assessed in cervical  tumor tissue and cervical non tumor  by immunohistochemistry. Method: This study was carried out on 30 patients with hisopathologically confirmed primary cervical cancer in addition 20 cervical tissues from control individuals with no cancer as control groups. From each patients and control cervical tissues were proposed for chromogen in situ hybridization (CISH) techniques to investigation the presence of HPV (HPV 16 /18) DNA CISH in cervical tissues and for immunohistochemistry was used to detected expression of p63. Results:P63 expression was found in 22/ 30 (73.3%) tumor sample while negative cases in 8/30 (26.7 %) tumor sample and no expression of p63 in cervical control groups. In this study demonstrated that cervical carcinoma cases which positive for HPV (16/18) which represented 26/30 from the cases also positive for p16 expression in 20 (76.9%) and negative for p63 expression in 6 (23.1%) .Conclusion: High expression of p63 in cervical carcinoma indicated a neoplastic progression  In addition, immunohistochemical detection of  P63 may be used to differentiate normal from malignant tissue in the clinical setting.