Study the Effect of Human –Derived Probiotic Bacteria on the Viability of Cryptosporidium Parvum

Abstract

This study was designed to evaluate the ability of Lactobacillus acidophilus probiotic bacteria to infect Cryptosporidium parvum viability by different bacterial dilutions and status. Cryptosporidium from stool samples of diarrheal patients were collected and diagnosed by parasitological methods, then molecular methods and cultivation in cell lines were done (Human adenocarcinoma cell). Probiotic bacterial dilutions (1:1, 1:2 and 1:3) and status (supernatant, pellet and dead bacteria) in De Man Rogosa Sharpe (MRS) mediu, the treatment of Cryptosporidium Parvum by adding Lactobacillus acidophilus dilutions and status were then incubated at incubation periods (24 hr, 48 hr, and 72 hr), detection of the Cryptosporidium count was carried out microscopically, and the parasite’s viability by MTT and flow cytometry technique. The results showed that Lactobacillus acidophilus has a great ability to treat Cryptosporidium parvum by inhibiting the activity and decreasing the count with highly significant differences.