The beneficial role of the probiotic Bacillus clausii on induced Murine Colitis: a clinical and pathological study

Abstract

This study was performed to investigate inflammatory bowel disease (IBD), especially ulcerative colitis in a murine model by using TNBS-induce colitis; therefore it was performed on 60 animals divided equally into five groups, the first group was the control which received standard food and water only, the second group was probiotic which used 100 μl of Bacillus clausii, the third group disease group by TNBS induced colitis, the fourth group probiotic with TNBS induce colitis orally, the fifth group probiotic with TNBS induce colitis intra-rectal; the results showed loss of body weight and increased in body temperature in disease group TNBS compared to control groups during the duration of the research, the macroscopical features showed the shortness of colon in length, as well as swollen and congestion of colon in the disease induced group compared to other control and probiotic groups; the micropathological results of colon showed sloughing of mucosal layer, sever hyperplastic goblet cells of the mucosal layer of the colon by PAS stain in the disease induced group compared to other control and probiotic groups. the current study concluded that whether Bacillus clausii can prevent TNBS-induced colitis which is associated with microbial dysbiosis and sought to understand its origins; as a consequence, probiotic treatment reduced colonic inflammation and By increasing a group of gram-positive bacteria, researchers reduced the microbial dysbiosis that TNBS Induce-colitis causes.