Evaluation of the efficacy of extracts of two types of Mollusca to control the larvae of the greater wax moth Galleria mellonella (lepidoptera pyralidae) by contact

Abstract

Extracts of two types of molluscs were used, one of which is the ethyl alcohol extract of the soft tissues of the oyster  Psudontopsis euphraticus (Burginat, 1852) and the aqueous extracts of its shells and their mixture under concentrations (10, 20, 30 and 40) mg.ml^-1, with the GC-MS and X-ray fluorescence technology for extracts. The other one is a commercial preparation which is a protease enzyme of Achatina fulica snail under concentrations (2.5, 5, 7.5 and 10) mg.ml^-1, to find out the effect of the two species in controlling the larvae of Galleria mellonella (L.) and to compare the results using a new insecticide to control insects of the Lepidoptera known as Belt_480SC (Flubendiamide) at concentrations (0.1, 0.2, 0.3 and 0.4) ml.L^-1.

The  results recorded a mortality rate of (100)% for the first instar at all concentrations, while the death rates differed for 4th and 7th instars. In the treatment of the 4th instar, soft-tissue extract exceeded the mortality rate of (100)% at a concentration of 40 mg.ml^-1, then the Belt_480SC and mixture treatments with close values (86.66, 76.66)% respectively, while the shell extract and protease treatments ranked third with close values (36.66 and 43.33)% respectively after 120 hours at the highest concentrations, while the 7th instar, it was noticed that when the two treatments with (soft-tissue extract and Belt_480SC) were equivalent to (70.00)%, while treatment with the mixture and protease ranked second with the effect of (43.33 and 26.66)% respectively, While the treatment with shell extract decreased by (10.00)%.