Gene Polymorphism of Phosphodiesterase 8B and its association with some biochemical variables that contribute to thyroid disorders

Abstract

The aim is to describe the polymorphism of PDE8B gene that some of which are related to TSH levels in blood. The study focusses on the polymorphism of phosphodiesterase 8B (PDE8B cAMP) gene and its correlation with some biochemical variable for hyperthyroid patients. The polymorphism of PDE8B gene, levels of Enzyme and the thyroid hormones of the serum have been studied for the hyperthyroid patients (thyrotoxicosis) in Iraq. The polymorphism of (PDE8B)rs(rs4704397) has been studied for 60 people who are suffers from hyperthyroidism and 30 of healthy people. The genotyping for all sample has been determined by using SNP genotyping methods. The levels of Thyroid hormones in blood have been measured by RIA radioimmunoassay. The levels of T3 and T4 for the patients group were higher than those with the healthy group with a decrease in the concentration levels of TSH. The genotyping and the allele frequencies for PDE8B gene between the patients group and the healthy group weren’t so much different. Comparing to the healthy group there was no correlation among the percentage, the allele frequencies and the genotypes of (PDE8B) with the Patients. The concentrations of the hormones levels also have been studies as per the genetic structure of PDE8B (cAMP) for the mutated genotypes compared to the normal genotypes in comparison with the normal Allele. The data shows the spread of hyperthyroidism with women (especially in the cases of pregnancy or confinement) more than with men. The disruption and the metabolism of the thyroid hormones that caused hyperthyroidism by the effects of the enzymes that encode by PDE8B gene.