Antibacterial and Anticancer Activities of (Free and Immobilized) Elastase Produced by Klebsiella Pneumoniae

Abstract

Elastase is a type of protease that specifically degrades elastin. The elastase produced by Klebsiella pneumoniae isolates and purified by three steps ammonium sulfate precipitation, ion-exchange chromatography, and Sephadex G-150 chromatography. The optimal condition for elastase production was showed high specific activity with starch (3.8 U/mg protein), casein as nitrogen source with a specific activity reached to (3.3 U/mg protein). The maximum elastase production was obtained when the pH value was (7.5) with specific activity (4.4 U/mg protein). Elastase (free and immobilized on TiO2- NPs) used in application as antibacterial and anti-cancer, and results showed high antibacterial activity against pathogenic isolates especially Lactobacillus acidophilus and Pseudomonas aeruginosa were affected by immobilized elastase. Free and immobilized elastase have anti-cancer activity against lung cancer using A549 cell line, and immobilize elastase was potent cytotoxic effect on A549 cells with IC50 142.8 µg/ ml compared with IC50 of normal cell line HdFn on 655.0 µg /ml.