Muhammad Aslam Chandio Mushtaque Ali Shah Sirichand Taj Muhammad Laghari Amanullah Lail Omparkash


Background: Neonatal mortality and morbidity in well-developed countries are commonly caused by neonatal septicemia. The pediatricians will decide related to the empirical treatment of neonates by using their susceptibility and their knowledge of microbial flora.Objective: The major reason for conducting this research was to study those neonates who were diagnosed with suspected neonatal sepsis and evaluate the antimicrobial susceptibility of blood culture isolation in them.Study design: A cross-sectional studyPlace and Duration: This study was conducted at Shaheed Mohtarma Benazir Bhutto Medical College Lyari Karachi from June 2021 to June 2022Methodology: This is laboratory-based research which was conducted in our hospital. All the patients were diagnosed with suspected neonatal sepsis and were admitted to the department of pathology of the hospital. The patients underwent sensitivity and blood culture testing. The disk diffusion method was used to carry out the antimicrobial susceptibility testing of blood culture isolation. This test was conducted using the guidelines provided by CLSI (Clinical Laboratory Standard Institute).Results: A total of 200 samples were collected out of which some were excluded and the rest were from 46 males and 30 females. The average age calculated was 13 days. The age ranges from 1 day to 28 days. E. coli (n=10), S. aureus (n=28), Acinetobacter spp. (n=8) and K. pneumoniae (n=9) were the common isolates. Methicillin-resistant Staphylococcus aureus (MRSA) constituted 25 percent of positive isolates. 6 patients showed pseudomonas aeruginosa isolation. The most effective antimicrobial against isolated gram-positive was linezolid. Linezolid showed 93.7 percent sensitivity.Conclusion: S. aureus, E. coli, and K. pneumonia are the major microorganisms that cause neonatal sepsis in our hospital.

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Bacteria, sepsis, newborn, infections

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