Study of Some Immune and Blood Markers Associated with Infection with the Intestinal Parasite Entamoeba Histolytica in Diabetic Patients

Abstract

This study was conducted on patients visiting hospitals in city and suburbs of Najaf Governorate for the period from the beginning of (2022/10/1) to (2023/2/1) to determine the relationship between the initial intestinal parasites with diabetics by identifying some immune indicators (IGM, IGG, IGA) and the White Blood Cells, Erythrocetes sedimentation rate, hematocrit (packed cell volume), Haemoglobin, and Biochemicals traits (Blood Sugar, Hemoglobin A1C, serum creatinine, uric acid, High Density Lipoprotein–cholesterol, cholesterol, C- Reactive protein and Total Serum Bilirubin. This study included (157) samples, including (40) feces of people from the people who were proven that they were affected by parasites only, which were tested to determine some epidemic indicators of the parasite, including age and sex, and (17) blood sample for the people who were associated with their affliction by Entamoebia histolytica and disease Diabetes together, and (50) blood sample for people with diabetes only, in addition to the control sample (50) blood sample of healthy people. As for those infected with the Entamoeba histolytica parasite, the highest infection rate was recorded in females and the lowest in male , with a significant difference as the infection rate was (75%) (25% ) respectively , as for the age group the highest infection rate was in the age group 1-10 years old, with a high significant difference, and the lowest infection rate in the group (21-40) years old, which amounted to (32.5 % )(25%) respectively . . The results also indicated that infection with the E. histolytica parasite and diabetes mellitus together were higher in females than in males, reaching (60.6%), (29.4%). The results of the current study showed about standards blood for people infected with parasite E.histolytica parasite and diabetes mellitus to Height in the concentration of Wbc and ESR and a decrease in the concentration of hematocrit and hemoglobin, which amounted to( 9.7 cells / mm) , (30 mm / hr) , (42.7%) , (13.3 g / dl) , compared to control. which resulted in (7.5 cells/mm) , (20 mm/hr) , (43%) , and (14.5 g/dl) , respectively. On the other hand, only those with diabetes showed an increase in the concentration of white blood cells and a decrease in the concentration of hematocrit, hemoglobin and erythrocyte sedimentation rate. Proportions were (7.9 cells / mm) , (43 %) , (14.5mg/dl) , (20mm/hr) The results for the biochemical parameters of patients with E.histolytica parasite and diabetes mellitus showed an increase in the level of glucose, cumulative sugar, C-reactive protein and urea, a slight increase in the concentration of bilirubin and a decrease in the concentration of cholesterol, high-density lipoproteins and uric acid, with significant differences which amounted to (216 mg/dl) ,( 7.9% ),(13.1 mg/dl) , (40mg/dl) ,(1.05 mg/dl) ,(130mg/dl) ,(38mg/dl) , (3.4mg/dl) respectively compared to the control (93mg/dl) , (5.5%),(5.5mg/dl) ,(30mg/dl) ,(1mg/dl) ,(160mg/dl) ,(57.3mg/dl ) , (4.3 mg/dl) respectively . As for diabetic patients only, there was a rise in glucose, glycated hemoglobin, C-reactive protein, high-density lipoproteins, uric acid level, cholesterol, urea, and a decrease in creatinine concentration, while the bilirubin concentration was within the normal level and the values were (213 mg/dl) ),( 8.3%) ,(6.6 mg/dl) ,(6.4mg/dl) ),6.6 mg/dl) ) , (171 mg/dl) (34.2mg/dl) ) (,0.9 mg/dl) ,(1mg/dl) respectively . Compared to the control range (93mg/dl), (5.5%), (57.3mg/dl) (5.5mg/dl), (4.3mg/dl), (160mg/dl), (30mg/dl), (0.95 mg/dl), (1mg/dl) The results showed that regarding the immune parameters of those infected with parasite and diabetes mellitus to decrease in the level of IgG and IgA and an increase in the level of IgM and the values were in the sick (1020mg/dl), (120mg/dl), 141 mg/dl) respectively compared to the control group (1150) (mg/dl, (235mg/dl), (135 mg/dl) respectively. In people with diabetes only, the study recorded a decrease in the concentration of IgA and IgM and an increase in IgG and the values were (221 mg/dl) (92mg/dl ), (1206 mg/dl) respectively. Compared to the control range (235 mg/dl), (135 mg/dl) and (1150 mg/dl) respectively