Kahlaa Muhammed Abbas Al-Mulla Abbas Shaker Al-Muhanna


Background: Enterobacter cloacae is also an important nosocomial pathogen responsible for bacteremia and lower respiratory tract, urinary tract and intra-abdominal infections, as well as endocarditis, septic arthritis, osteomyelitis and skin and soft tissue infections. Introduction: Acute cholecystitis (AC) is one of the most common surgical diseases. Bacterial infection accounts for 50% to 85% of the disease’s onset. Since there is a close relationship between the biliary system and the gut, the aims of this study were to characterize and determine the influence of gut microbiota on AC, Methodology: 174 clinical specimens (swab) were collected from patient suffering from gall-bladder infection, after the specimens were cultured on suitable media, the identification of bacterial isolates was carried out using biochemical test and Vitek-2 system, PCR amplification technique were used to investigation the predominance, aggregation genes csgA and csgD and adhesion genes fimA and fimH among E cloacae isolate Results: After incubation period the results appear that (47.12%) of specimens were gave bacterial growth and (52.88 %) were appear no growth. E cloacae were identified in (24.39%) of bacterial growth., the results PCR multiplication technique appear that (40%) and (10 %) of isolates carried csgA and csgD genes, while (15%) and (35%) of bacterial isolates were carrying fimA and fimH genes. Conclusions: Bacteria the major agent of gall-bladder infections and E cloacae was the most common bacteria causing gall-bladder infections.

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Enterobacter cloacae, csgA , csgD, Gallbladder, virulence factors

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