Evaluating the relationship of serotonin and ferritin levels with (P53) gene expression in Beta thalassemia patients

Abstract

Objective:Beta thalassemia is an inherited blood disorder that affects the synthesis of the beta chain of hemoglobin, resulting in production of deficient RBCs. Iraq is among the countries where this disorder is prevalent. Beta thalassemia patients need frequent transfusions of healthy blood throughout life in order to mitigate the symptoms and can suffer from iron overload complications as a result . Serotonin is a neurotransmitter also considers a hormone is a natural chemical that intestines and brain produce. It helps the body send messages between nerve cells and affects mood and emotions. P53 the tumor protein is a regulatory protein that is often mutated in human cancers. P53 has been described as "the guardian of the genome" because of its role in conserving stability by preventing genome mutation. Aim:The aim of this study is to determine the relationship of serotonin and ferritin levels with (P53) gene expression in transfusion dependent β-thalassemia major patients. Methods:The present study is conducted at Thalassemia hematology center in Al-Kut women &children Hospital in Wasit province / Iraq. The study involve 85 samples, 55 specimens of β-thalassemia major patients and 30 blood samples as control. Measurements of serotonin and P53 was by using enzyme-linked immunosorbent assay (ELISA). Ferritin was measured automatically by Cobas e 411 device . Results:The results of the present study revealed reduced serotonin level in β-TM patients and shown a significant correlation (P≤0.02) in patients when compared with control. Serum ferritin showed a major increased level in blood transfusion dependent β-TM patients and its presented significant correlation (P≤0.001) in patients group when compared with control group. P53 showed increased level in β-TM and it’s correlated positively in patients (P≤0.01) when compared with control. Serotonin and P53 showed no significant correlation(P>0.05) with ferritin level when compared between the same group of β-thalassemia patients.