Immunogentic Detection of Human Herpes Virus-6B, IL4and TNF-α in Male Patients Suffering from Infertility Disorders

Abstract

The study aims to investigate HHV6-B, IL4 and TNF-α in infertility male patients. To achieve this goal, 200 samples (blood and semen to each patient) were collected from infertility center in ALSadr hospital and outside laboratory in Najaf Governorate (160 patients and 40 from normal person as control group). Patients were married and infertile. Samples were collected from patients during the period from 1 of December 2020 to 15 December 2021.

Polymerase chain reaction (PCR) technique was used to detect the occurrence of HHV6-B DNA among infertility male patients. Molecular assay results of this study revealed the presence of HHV6-B was 17 (10.63%) in infertility male patients. HHV6-B DNA not detected in seminal fluid of healthy persons.

The present study has detected that single nucleotide Polymorphisms (SNP) in IL-4 -590 C/T genotype frequency distribution in infertility male patients. The genotypes relative frequency in patients were as follow: CC (33.75%), CT (43.75%) and TT (22.5%) among them. C allele is higher frequency (65.32%) in patients than T allele (34.67%) in which there was no significant difference from statistical perspective (P value = 0.004) among infertility male patients and control group. The genotypes relative frequency in HHV-6B infections was: CC (29.41%), CT (41.17%) and TT (29.41%). There was no significant difference (P value = 0.849) among patients.

Also, in this study has detected that single nucleotide Polymorphisms (SNP) in TNF-α -308 G/A genotype frequency distribution in infertility male patients. The genotypes relative frequency in patients were as follow: GG (26.25%), GA (45.63%) and AA (28.13%) among them. G allele is higher frequency (56.2%) in patients than A allele (43.8%) in which there was no significant difference from statistical perspective (P value = 0.212) among infertility male patients and control group. The genotypes relative frequency in HHV-6B infections was: GG (23.53%), GA (52.94%) and AA (23.53%). There was no significant difference (P value = 0.9739) among patients.