Preparation, Characterization, Theoretical and Biological Studies of Mixed Ligands Complexes Derived from Heterocyclic Compound Against Different Types of Bacteria and Fungi

Abstract

New Mannich Base has been prepared from condensation reaction of 2-chloro. -N-(5-mercapto-1,3,4-thiadazole-2-yl) acetamide with piperidin(L) and drop of formaldehyde. The Resulting compound represents Mannich base (2-Chloro-N-(5-(piperidin-1-ylmethylthio)-1,3,4-thiadiazole-2-yl) acetamide (L₎. A series of mixed ligand complexes was prepared from (L) and1,10 phenotheroline with the metal ion Co (II), Ni (II), Cu (II), Pd (II), Pt (IV) and Au (III). The compounds obtained were successfully characterized by different spectroscopic techniques such as C.H.N.S, FTIR, UV-ViS, 1HNMR, 13CNMR, magnetic sensitivity and molar conductivity. The ratio of M: L was measured by using molar ratio method in solution state. Experimental results showed that the mixed ligands coordinated as tri-dentate from Mannich base (L) and bi-dentate from (1,10 phenotheroline). The antibacterial and antifungal activity of ligand and complexes was studied against two types of bacteria and one type of fungi at 0.02M. The standard heat of formation and binding energy of the ligands and all prepared complexes were calculated using the Hyper chem 8.0.7 program, the study proved that the complexes are more stable than the ligands. The vibrational frequencies of the ligands were calculated, and the practical and theoretical results were compared, in addition to calculating the error ratio between the two methods. Keywords: Transition Metal Complexes, Mannich Base, Mixed ligands, 1, 10-Phenanthrolin, Antibacterial and Antifungal activity