Study the expression of MicroRNA -22 and its correlation with some hormones in samples of Iraqi infertile men

Abstract

Infertility is defined as a couple's inability to conceive and reproduce after a year of regular coitus without contraceptive methods, which can be caused by a variety of physiological and genetic factors. has-MiRNA-22  have been mention in several studies that may lead to male infertility. In this study we will try to evaluate role of and has-MiRNA-22 in idiopathic male infertility by Estimate expression level of has-MiRNA-22 in semen samples of infertile male compared with fertile male semen samples, Analyzed some male hormones levels (estrogen-testosterone-FSH-LH) in both infertile and fertile Iraqi men and Study the association between the gene expression of has-MiRNA-22 gene with hormones levels in these samples. This study was conducted at the University of Baghdad Institute of Genetic Engineering and Biotechnology for Postgraduate Studies from 1 November 2021 to the end of May 2022. A 100 semen and blood samples patients were taken from the Kamal Al-Samarrai infertility treatment Hospital in Baghdad. A total of 75 samples of semen from infertile men as patient and 25 samples from fertile men were collected as a control. Patient group categorized to three subgroups as following: asthenozooospermia, oligoasthenozooospermia and azoospermia. The RNA of has-MiRNA-22 gene was extracted from seminal plasma and Real time PCR was used to quantitatively estimate its expression level. The microscopic semen analysis indicated highly significant (p<0.05) variations in semen parameters between Study groups while the duration of infertility show a Non-significant associated with infertility. There is a non-significant positive correlation between (FSH-Testosterone-LH) hormones and Micro RNA 22 5P expression levels which means increase the expression level of MiR-22-5p will have a positive effect on those hormones while the (E2) shows a non-significant negative correlation with the expression level of MiR-22-5p.