Evaluation Antifeedant and Larvicidal Effects Of water plant Ceratophyllum demersum Extracts, Against Plodia interpunctella Larvae

Abstract

To producing bioinsecticides from environmentally friendly natural products .The present study was designed to producing bioinsecticide from water plant Ceratophyllum demersum extracts against the third and fifth larval instar of the Indian meal moth Plodia interpunctella based on LC50 values after 24, 48 and 72 hours of exposure, using the insecticide Nestor as a comparison . The results showed that the ethanolic extract of the C. demersum was toxic to the larvae, as its toxicity was superior to Nestor, as the LC50 values of the third larval instar were 0.051, 0.038 and 0.020 μl/insect, , , and they were 0.094, 0.038 and 0.48 μl/insect, after 24, 48 and 72 hours respectively. The results of testing the biological effects of ethanolic extracts on the external appearance or histological structure of the body wall and the mid gut as the most important target tissues for the resistant to insecticides in superficially treated insects exposed to feeding media treated with alcoholic extracts proved that these extracts It caused feeding inhibition, and consequently the death and death of treated insects after 10, 20 and 30 days of treatment. Accordingly, a number of effective phenolic compounds found in plant extracts were identified, the most important of which were Caffeic acid, catechin, Ellagic acid, Gallic acid, Kaempferol, p-Coumaric acid, Quercetin and Vanillic acid Using HPLC technology, the presence of these compounds in the plant was found, and the percentage of the two compounds was Quercetin and Kaempferol was the highest, as Quercetin appeared in the crude ethanolic extract (49.8%) and Kaempferol in the crude aqueous extract (66.2%)