Background/aim: Acetaminophen (APAP) overdose results in severe liver damage that may develop into acute liver failure. The goal of this study was to investigate the effect of acetaminophen on liver function due to toxic doses of acetaminophen. Methods:A total of 30 growing rats were divided into two equal groups, 10 rabbits each. The first group control dministered with normal saline and the second group paracetamol -treated rats) for 30 days. Blood samples were withdrawn to measure serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), protein, and bilirubin activities were assayed.Results:In paracetamol -treated group, significant increases in aspartate aminotransferases,and alanin aminotransferases activities, and significant decreases in catalase bilirubin activities levels were determined compared to the control group.Conclusion: paracetamol administration produces noticeable biochemical changes in a dosedependent manner associated with increased liver enzyme.