The Effect of ZnO NPS and TiO2NPs on Gene Expression of Five adhesion genes fimB - fimH of E. coli Isolated from Clinical Sources in Mousl –Iraq

Abstract

The study was carried out on (135) various clinical samples collected from patients attended Ibn Sina and Al Salam Hospitals in Mosul city during December 2020 – March 2021. E.coli isolated from urine and burns at percentage 20% for each of them shown.where isolation from sputum at 10 % and from stool (control) at 100. The sub-Minimum Inhibition Concentration (sub-MIC) of ZnO NPs for E. coli it appeared between (2500–1250) µg/ml, while for TiO₂ NPs appeared between (5000–2500 )µg/ml.The present of adhesion genes in (24) isolates of E. coli was as follow: fimA at percentage 8.3% and fimB, fimC present at 100% for each one, fimE, fimI as 95.8% for each one, fimH 87.5%.Real Time PCR used to detect the ability of ZnO NPs and TiO₂ NPs on expression of the adherence genes fimB ,fimC, fimE, fimI and fimH. In E.coli show increasing in expression after exposed to sub MIC of ZnO NPs at concentration (2500µg/ml), the most effected gene was fimH followed by fimB and fim I gene while same effect on fimC and fimE. E.coli show increasing in expression after exposed to sub MIC of TiO₂NPs at concentration (5000µg/ml), the most effected gene was fimC followed by fimH and same effect on fimB and fimI and less effect on fimE