Cytogenetic Effect of Cicer Arietinum Ethanolic Extract on the Male Rats Treated with Sorafenib

Abstract

The present study was conducted to estimate the ameliorative effect of Cicer Arietinum ethanolic extract against cytogenetic effect of Sorafenib in male rats. Twenty-four adult male rats were divided into four groups each group contain seven rats; G1(control negative group received distilled water along of period of study), G2(control positive group received 200mg of Sorafenib only), G3(received Cicer arietinum extract at dose (314.9 mg/kg) and G4 group treated with Sorafenib at dose (200mg/kg/B.W.) and Cicer arietinum extract (314.9mg/kg). The results of chromosomal aberration showed significant P≤0.05 increase in Chromosomal Aberration of G2 group as compared with all groups. While Acentric%, Polyploidy%, Ring% of G3 treated group showed no significant difference (P≤ 0.05), as compared with G1 group. Furthermore, the Acentric% of G4 group showed a significant decrease (P≤0.05) as compared with all other treated groups. Whereas Mitotic Index revealed significant P≤ 0.05 increases in animals exposed to 200mg/kg.bw when compared with groups of plants and control negative group Also, the DNA fragmentation revealed significant P≤ 0.05 increases in fragmented DNA of animals G2 compared with G1 and G3. In conclusion, Cicer arietinum seeds ethanolic extract contain potential source of polyphenolis demonstrated biochemical and functional properties against cytogenetic effect of chemotherapy drug Sorfenib. Moreover, it showed DNA damage protection.