Alloxan-Induced Oxidative Stress and Its Impact on the Reproductive System in Male Albino Rats and the Protective Role of Malva Parviflora Seed Extract

Abstract

The current study aimed at evaluating the impact of the protective role of the aqueous extract of Malva parviflora seeds to reduce oxidative stress induced by the use of Alloxan on the male reproductive system and on a number of biochemical and histological variables and sperm parameters and comparing the protective role of the extract with Insulin  Clargine, the study used 35 rats distributed in plastic cages in seven groups, 5 rats for each group, which included the first group, the healthy control, the second group was the control group, which was injected subcutaneously with Alloxan at a concentration of 130 mg/kg, the third group, the healthy control, which was treated with aqueous extract of Malva parviflora seeds at a concentration of 100 mg/kg by tube feeding every 24 hours, the fourth healthy group was treated with Insulin  Clargine at a concentration of 5 IU/kg subcutaneously every 48 hours, the fifth group was injected with Alloxan at a concentration of 130 mg/kg subcutaneously, which was treated with extract of Malva parviflora seeds orally at a concentration of 100 mg/kg every 24 hours, the sixth group was injected with Alloxan at a concentration of 130 mg/kg subcutaneously, which was treated with Insulin  Clargine  at a concentration of 5 IU/kg subcutaneously every 24 hours, and the seventh group was injected with Alloxan at a concentration of 130 mg/kg subcutaneously and treated with Insulin drug Clargine subcutaneously at a concentration of 5 IU/kg every 24 hours with the aqueous extract of Malva parviflora seeds at a concentration of 100 mg/kg every 24 hours. The results showed that the group of rats in the infected control had a significant decrease at the probability level ((p≤0.05) in the concentrations of each of the interstitial cell stimulating hormone (ICSH), the sperm hormone (SSH) stimulating hormone and the testicular saturation hormone Testosterone and a decrease in the concentration of glutathione, superoxide dismutase (SOD) and catalase (CAT) enzyme, a decrease in sperm parameters represented in the number of concentration and motility, and an increase in the number of distorted ones compared to the control group. Histological sections of the testes also showed the presence of necrosis in the testicular tissues, as well as unclear stages of sperm formation and a decrease in the number of mature sperms. As for the diabetic group treated with the aqueous extract of Malva Parviflora seeds, the diabetic group treated with Insulin Clargine, and the group with the disease treated with the aqueous extract of the Malva Parviflora seed with Insulin Clargine, they showed an improvement and an increase in the concentrations of SSH, ICSH, Testosterone and the antioxidants SOD, and CAT and GSH, and the decrease in lipid peroxidation (MDA) in the blood serum, as well as the improvement of the parameters of the sperm represented by an increase in the number and motility, and the number of abnormalities decreased, and the histological sections showed a clear improvement in the testes tissues.