Accelerating Burn Wound Healing by Egg Yolk Oil Extracts Against Pseudomonas Aeruginosa Biofilm Infection

Abstract

Biofilm can obstruct wound healing through a variety of mechanisms; the National Institutes of Health and the Centers for Disease Control and Prevention estimate biofilm is to blame for 65% to 80% of infectious diseases in humans. This study hypothesis was established through an experimental, laboratory-prospective study to assess burn wound healing if treated with egg yolk oil extracts against Pseudomonas aeruginosa biofilm infection, taking into account the importance of biofilm in postponing burn wound healing. Through using the microtiter plate method, the formation of biofilm was investigated in P. aeruginosa clinical isolates from patients associated with burn infections. Yolk oil extracts were evaluated in vitro for minimum inhibitory concentration (MIC) and antibiofilm inhibitory concentration against the strongest biofilm producer designated as (PA12) and (ATCC 27853) isolates. Our findings indicate that the MIC was determined at 45 mg/ml and 60 mg/ml respectively. In the biofilm inhibition assay, a sub-inhibitory concentration (SIC) of 30 mg/ml was screened, resulting in a remarkable statistically significant reduction in biofilm quantity. The (SIC) was evaluated in vivo against an established P. aeruginosa biofilm infection on a conventional Fischer F-344 rat burn wound model. Histological examination of wound healing in burned rats treated topically with yolk oil extracts revealed extensive re-epithelialization and anti-inflammatory benefits without tissue scarring.