Effect of Lactobacillus acidophilus and Pediococcus pentosaceus on sigA gene expression of Shigella sonnei

Abstract

The study aimed to evaluate the inhibitory role of probiotics on Shigella sonnei in molecular level. ‏Twenty five stool specimens were collected from children under 5 years suffering from bloody acute diarrhea hospitalized in Al Zahrawi Private Hospital, Mosul city from July 2021 till September 2021. One isolate of Sh.sonnei was obtained from these specimens with incidence rate of (4%), it was identified according to cultural and morphological as well as biochemical characteristics measured by Vitek 2 System. Tow probiotics were used in this study included Lactobacillus acidophilus and Pediococcus pentosaceus. Treatment of local isolate Sh.sonnei with sub-MIC cell free cltuture supernatant of the probiotics L.acidophilus and P.pentosaceus led to down regulation of the gene sigA which was tested by Real Time Quantification PCR.