The anti-oxidant and DNA protective effects of Cicer arietinum ethanolic extract in comparison to rosuvastatin in Triton X-100 treated female rats

Abstract

The current study aimed to detect of the protective effect of the Cicer arietinum seed extract to enhancing of anti-oxidant capacity and anti-inflammatory activity with DNA protectant effect in comparison with rosuvastatin in animals subjected to oxidative stress by triton X-100. Thirty female rats were equally separated into five groups and treated for (28 days). The first group termed G1 treated with distilled water which was negative control group, the second one G2 treated with triton X-100 at dose (100 mg/kg) I.P positive control group, the third group G3 treated with Cicer arietinum crude extract at dose 349.015 /kg B.W after induction with triton X-100, the fourth group G4 treated with rosuvastatin after induction with triton X-100. And the last group treated with combination of Cicer arietinum extract with rosuvastatin after induction with triton X-100.the result of anti-inflammatory ability measured using interleukin-6 (IL-6). The result of IL-6 of both G3 and G4 treated groups revealed a significant decrease in comparison with G2. while there was no significant differences between G3 and G4 while G5 showed significant decrease comparing to all treated groups. Additionally the result of glutathione peroxidase (GPX) and super oxide dismutase (SOD) revealed concentrations in G3 and G4 were significantly higher than in G2.While G5 showed significant decrease during comparing with all treated group, with no significant differences with G1.Furthermore the result of DNA-fragmentation commit assay inG2 showed significant increase in DNA damage comparing to G1.While both of G3 and G4 showed significant decrease in DNA damage comparing to G2, but the G3 decrease in DNA damage when matched to G4.the result of G5 revealed significant decrease comparing to G2and G4.