Molecular investigation of NUC and MECC genes in S. aureus isolated from meat

Abstract

Background and Aim: S. aureus was a pathogen which caused numerous infectious diseases, in human and animal. The current study was directed to investigate the occurrence of S. aureus that isolated from meat, then PCR detection of nuc and mecC genes in all isolates of S. aureus. Materials and Methods: An entire of 100 meat samples were investigated. The S. aureus isolates after identified were subjected to PCR assay to detect the nuc and mecC genes in all isolates of this bacteria. Results: The occurrence of S. aureus was 40% (40/100) that isolated from meat samples. Out of 40 samples of S. aureus studied by PCR technique, the results revealed that 100% of isolates give positive results of nuc gene, while no one isolates had mecC gene. Conclusion: The using of nuc gene-based PCR was an accurate and quick method for detection the S. aureus.