Study the association between indothelin-1 with age and body mass index in women with poly cystic ovarian syndrome

Abstract

Polycystic ovarian syndrome (PCOS) is a common inflammatory endocrine disorder that affects women of reproductive age and considered risk factor for infertility. Endothelin-1 (ET-1) is an indicator of endothelial injury dysfunction and is elevated in women with androgen excess polycystic ovary syndrome (PCOS). Objective: this study aims to detect relation between endothelin-1 with age and bmi in pcos compare to control group. Subject and method: Case control study enrolled 120 women classified according to BMI (20-35) kg/m² and Age (20-40) years.60women diagnosed with PCOS by dr..Anfal AL.kaabi is agynecologist and by sonar and60 apparently healthy . PCOS cases were also classify to three subgroups (A, B, C) accroding to infertility (normal which no infertility, primary infertility was 40, secondary infertility was 20). The samples were collected in the morning fasting state from November 2020 to May 2021. Biochemical variables BMI calculated weight divided by square height in meter(km/m²). measure serum ET-1 (ng/ml) were assessed by ELISA. measure hormons (LH,FSH,F.testo) in cycle day two(CD2) by minividas .measure lipid profile (total cholestrol TC, high density lipo protrin HDL, low density lipoprotein LDL, very low density lipoprotein VLDL and triglyceride TG) by colorimetric assay using spectrophotometer. Measure insulin by ELISA assay. and fasting blood sugar by maglumi device. Results: elevated ET-1 obesity and increasing with age. The mean levels of biomarkers among infertility groups in women with PCOS shown that all parameters were significantly differed among the groups (p values <0.05) . level of LH; Testosterone; TG; Insulin; W\H and LH/FSH ratio were shown a positively significant increasing with secondary infertility cases. While FSH; HDL and fasting blood sugar were decreased significantly in the same group Conclusion: Endothelin-1 may be pre indicator diagnostic markers for PCOs -Endothelin was increasing with age; negative relation between ET-1 and HDL which is may be indicator for heart disease.