Effect of the coelomic fluid of the earthworm Aporrectodea tuberculate in inhibiting the growth of different types of pathogenic bacteria

Abstract

Earthworms Aporrectodea tuberculate that are characterized by their large size, pale pink or gray color, in this study, the coelomic fluid of this worm was used on different types of pathogenic bacteria (Staphylococcus aureus, Pseudomonas aerugniosa, salmonella typhi, Klebsi ella pneumoniae and E.coli) using two solvents Petroleum Ether and Acetone. The results showed that the coelomic fluid dissolved with Petroleum Ether was more effective than the coelomic fluid dissolved with acetone when it was treated with Salmonella bacteria and its inhibitory activity was estimated at 15.67 mm compared with acetone which recorded an inhibition diameter at the same bacteria 13.67 mm while the AM antibiotic gave an inhibitory activity of 25.00 mm with significant differences between the treatments, there was no significant difference in the inhibitory activity on Staph and Pseudo bacteria between the two solvents Petroleum Ether and acetone and their effectiveness was estimated by mm, respectively, 14.00 and 13.67 for Petroleum Ether and 12.33, 13.67 for acetone, and the antibiotic did not give any activity against the precursor bacteria. While in Klebsiella bacteria, the inhibitory activity of the anti-AM was 13.00 mm very close to that of the dissolved coliform liquid Petroleum Ether, which gave an inhibition rate of 13.33 mm. Its growth inhibition was 12.33 and 11.67 mm, respectively, which was higher in both of them than the AM antibiotic, which recorded an inhibition rate of 10.00 mm.