In Vitro Anticancer Activity of Different Fractions of Bombax Ceiba Plant Cultivated in Iraq That Compared with Vincristine as A Positive Control

Abstract

The bombax genus is a large and taxonomically complex one that consists of different species present in Asia, Mediterranean Australia, and southern Asia regions. This study is concerned with the extraction, fractionation, and estimation of some known biologically important constituents from the plant extraction of these constituents was carried out by Soxhlet apparatus, the active constituents according to the differences in their polarities using, ethyl acetate and hexane. The second part is the assessment of the possible anti-cancer activity of the hexane-ethylacetate fractions This is done by anti-cancer activity on the MCF7, AMJ13 cell lines using different concentrations of extracts starting from 10µg to 300 µg and comparing the effect of extracts with the positive control (vincristine) The results showed that the hexane, fraction from the plant show inhibition of the growth of cancer in a manner showed that significant of the experiment; (p˂0.05). After detection of the most active fraction of bombax ceiba detection of inhibitory concentration (IC50) was done one-way Anova test showed that hexane fraction has a more effective effect on cancer cells than ethyl acetate fraction when compared with positive control which was vincristine.