Effect of Lactobacillus Acidophilus and Pediococcus Pentosaceus on Inva Gene Expression of Salmonella Enterica Serovar Enteritidis (S.Enteritidis)

Abstract

The study aimed to evaluate the inhibitory role of probiotics on Salmonella Enteritidis in molecular level.Twenty five stool specimens were collected from children under five years suffering from acute diarrhea hospitalized in Al-Zahrawi Hospital,Mosul city from 1/7/2021 till 1/9/2021. One isolate of S.Enteritidis was obtained from these specimens with incidence rate of (4%),it was identified according to cultural and morphological as well as biochemical characteristics measured by Vitek 2 system. Two probiotics were used in this study included Lactobacillus acidophilus and Pediococcus pentosaceus. Treatment of local isolate S.Enteritidis with sub-MIC cell-free culture supernatant of the probiotics L.acidophilus and P.pentosaceus led to down regulation of the gene InvA which was tested by RT-qPCR.